厚朴总酚对金黄色葡萄球菌的抑菌作用及抗溶血作用

doi:10.11843/j.issn.0366-6964.2018.02.023

Abstract

Abstract:

In order to investigate the antimicrobial activity and anti-infective activity of Total Magnolol on mastitis-related pathogens (mainly Staphylococcus aureus), a broth microdilution method was employed to determine the minimal inhibitory concentrations (MIC) of the Total Magnolol against S. aureus strains. Hemolysis assay, Western blot analysis, real-time RT-PCR assay and cytotoxic assay were further performed to evaluate the influence of this compound on S. aureus virulence. The results showed that the minimal inhibitory concentration of Total Magnolol against S. aureus were 8-16 μg·mL^-1. Furthermore, treatment with Subinhibitory concentration of Total Magnolol could inhibit the expression of α-hemolysin, reduce the pathogenicity of Staphylococcus aureus, and significantly alleviate the injury induced by α-hemolysin of Staphylococcus aureus.

CLC Number:

S853.7

LIU Gui-lan, ZHOU Yong-lin, LI Wen-hua, XIE Ling-ling, LIU Ai-ling, DENG Xu-ming. The Antimicrobial and Anti-hemolysis Effects of Total Magnolol on Staphylococcus aureus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 432-438.

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The Antimicrobial and Anti-hemolysis Effects of Total Magnolol on Staphylococcus aureus

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